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目的 ELISA检测HBsAg结果阴性的无偿献血者样本进行核酸检测，再对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测，分析是窗口期感染还是隐匿型乙型肝炎。方法使用双ELISA试剂检测无偿献血者样本，对检测结果阴性的无偿献血者样本使用浩源核酸筛查系统进行核酸检测，对核酸检测阳性结果样本进行“两对半”检测并对献血者追踪检测。结果 HBsAg(ELISA)阴性献血者血液样本12548份进行核酸检测，结果有13份样本检出HBV DNA，通过“两对半”检测以及对献血者追踪检测，确定2例为窗口期感染，11例为隐匿型乙型肝炎。结论核酸检测应用于血液筛查，防止输血传播疾病有及其重要的意义。“,”Objective ELISA in the detection of HBsAg negative blood donation sample nucleic acid detection, and of nucleic acid positive test results for samples of “two half and half” detection and the donors track detection, is to analyze the infection in window period or occult hepatitis B. Methods Double ELISA reagent testing samples of unpaid blood donors, negative unpaid donors samples using the result of the test grandsoo DNA screening system for nucleic acid detection, the nucleic acid testing positive results “two half-and-half” samples and blood donors track detection.Results HBsAg (ELISA) negative blood samples of 12548 blood donors for nucleic acid detection, the results of 13 samples detected HBV DNA, by “two half-and-half” detection and track testing of blood donors and to determine 2 cases for the window period, 11 cases of hepatitis b virus infection aslar-pacific perspective. Conclusion Nucleic acid detection applied in blood screening to prevent blood transfusion transmit ed diseases and its important significance.