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目的研究负载人NDRG2 2种亚型(长短2种亚型分别命名为NDRG2L、NDRG2S)基因对HO-8910细胞裸鼠皮下成瘤的作用;探讨NDRG2对顺铂(DDP)诱导卵巢癌HO-8910细胞凋亡的作用。方法实验组与空载体组卵巢癌HO-8910细胞接种裸鼠皮下,观察裸鼠成瘤情况;DDP处理携带人NDRG2基因的卵巢癌细胞株HO-8910,流式细胞术检测细胞凋亡。结果 NDRG2促进HO-8910细胞裸鼠皮下成瘤;NDRG2促进HO-8910细胞株抗顺铂(DDP)诱导的细胞凋亡。结论通过检测负载NDRG2的HO-8910细胞裸鼠体内荷瘤试验,进一步证实NDRG2可以明显促进卵巢癌HO-8910细胞的增殖;通过检测转染NDRG2及空载体组卵巢癌细胞在DDP作用下的细胞凋亡情况,提示NDRG2基因可能通过减少DDP诱导的细胞凋亡而诱发化疗耐药。
Objective To study the effect of NDRG2 on the subcutaneous tumorigenesis of HO-8910 cells in nude mice, and to investigate the effect of NDRG2 on ovarian cancer HO-8910 induced by cisplatin (DDP) The role of apoptosis. Methods The ovarian cancer HO-8910 cells were inoculated subcutaneously in nude mice in experimental group and empty vector group to observe the tumorigenicity of nude mice. DDP was used to treat ovarian cancer cell line HO-8910 carrying human NDRG2 gene and the apoptosis was detected by flow cytometry. Results NDRG2 promoted the subcutaneous tumorigenesis of HO-8910 cells in nude mice and NDRG2 promoted the apoptosis of HO-8910 cells induced by cisplatin (DDP). Conclusions NDRG2 can significantly promote the proliferation of ovarian cancer cell line HO-8910 by detecting the tumor-bearing activity of NDRG2-loaded HO-8910 cells in nude mice. By detecting the expression of NDRG2 in ovarian cancer cell line DDP Apoptosis, suggesting that NDRG2 gene may induce chemoresistance by reducing the apoptosis induced by DDP.