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对宾川烟草病区病毒病进行调查采样,应用病毒分离提纯技术、电子显微镜技术开展鉴定研究,得出:(1)宾川烟草病毒病原以CMV,PVY,TMV,TEV发生较为普遍,且危害严重,此外有PVX,一种球形病毒〔可能是番茄斑萎病毒(TSWV)〕也有零星发生,以症状来看,还有环斑、曲叶、丛枝、条纹等类型,但未分离到明显病原;(2)病毒病病原的分布在宾川主栽烤烟品种上没有明显的差异,而分布与生态类型有关,年度间种群分布相对稳定;(3)探明烟苗期烟苗带毒率和带毒量是宾川烟草中后期病毒流行的决定性因素;(4)探明了病毒、寄主、媒介(蚜虫)三者之间的关系及其烟草病毒病的侵染循环.
The investigation and sampling of virus disease in Binchuan tobacco ward, application of virus isolation and purification technology, electron microscopy technology identification research, draw: (1) Binchuan tobacco virus pathogen with CMV, PVY, TMV, TEV occurs more commonly, and the harm In addition, PVX, a spherical virus (may be tomato spotted wilt virus (TSWV)〕 also sporadic, in terms of symptoms, there are rings, leaves, branches, stripes and other types, but did not separate significantly Pathogen; (2) Distribution of pathogen of virus There was no obvious difference in the distribution of virus pathogen in Binchuan, but the distribution was related to the ecological type, and the distribution of population was relatively stable in the year; (3) And the lethal dose is the decisive factor in the epidemic of late-stage virus in Binchuan tobacco. (4) The relationship between virus, host, vector (aphid) and infection cycle of tobacco virus disease has been proved.