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目的:探讨新建成的体外受精(IVF)胚胎实验室挥发性有机化合物(volatile organic compound,VOC)的浓度变化及不同浓度VOC环境对小鼠体外受精和胚胎发育的影响,同时比较目前常用的去除实验室内VOC方法的效率。方法:测定胚胎实验室装修后不同时间段的VOC浓度变化,比较不同浓度的VOC对小鼠体外受精率、2-细胞率和囊胚率的影响;比较排风扇抽风机、Coda空气净化器和单纯活性炭包去除室内VOC的效率。结果:装修后第1个月(M1)VOC浓度为384.00 ppb,显著高于对照组(199.00 ppb)(P<0.05);装修后第2个月(M2)VOC浓度为279.50 ppb,与对照组相比差异具有统计学意义(P<0.05);装修后第3个月(M3)VOC浓度为210.25 ppb,与对照组(206.00 ppb)相比差异无统计学意义(P>0.05)。装修后1个月(M1)实验组体外受精率、2-细胞率和囊胚率分别为62.3%、71.5%、72.0%,显著低于对照组(91.8%、92.2%、93.0%),组间差异具有统计学意义(P<0.05)。装修后3个月(M3)时受精率、2-细胞率和囊胚率(91.5%、90.7%、91.4%)与对照组(89.8%、90.1%、92.5%)相比差异无统计学意义(P>0.05)。Coda空气净化器处理3个月后可去除室内60.15%的VOC,显著高于排风扇抽风(51.88%)和单纯活性炭吸附(28.95%)的去除效率。结论:实验室内高浓度的VOC会对小鼠体外受精胚胎造成严重的胚胎毒性,导致受精率、2-细胞率和囊胚率降低,胚胎质量下降和发育受阻;Coda空气过滤器是一种有效的去除实验室内VOC的方法。
OBJECTIVE: To investigate the changes of concentration of volatile organic compounds (VOCs) in fresh-in vitro fertilization (IVF) embryos and the effects of VOC in different concentrations on in vitro fertilization and embryo development in mice. At the same time, Efficiency of laboratory VOC methods. Methods: The changes of VOC concentration in different time periods after embryo lab decoration were measured. The effects of different concentrations of VOC on in vitro fertilization rate, 2-cell rate and blastocyst rate were compared. Exhaust fan exhaust fan, Coda air purifier and pure Activated carbon package to remove indoor VOC efficiency. Results: At the first month after decoration, the VOC concentration was 384.00 ppb, significantly higher than that of the control group (199.00 ppb) (P <0.05). At the second month after decoration, the VOC concentration of M2 was 279.50 ppb, (P <0.05). At the third month after decoration, the VOC concentration was 210.25 ppb, which was not significantly different from that of the control group (206.00 ppb) (P> 0.05). The in vitro fertilization rate, 2-cell rate and blastocyst rate in experimental group were 62.3%, 71.5% and 72.0% respectively at 1 month after decoration (M1), which were significantly lower than those in control group (91.8%, 92.2% and 93.0% The difference was statistically significant (P <0.05). There were no significant differences in fertilization rate, 2-cell rate and blastocyst rate (91.5%, 90.7%, 91.4%) compared with control group (89.8%, 90.1%, 92.5%) at 3 months after decoration (P> 0.05). After 3 months, Coda air purifier can remove 60.15% of indoor VOCs, which is significantly higher than that of exhaust fans (51.88%) and pure activated carbon adsorption (28.95%). CONCLUSION: High concentrations of VOCs in the laboratory cause severe embryotoxicity on in vitro fertilized mice, resulting in reduced fertilization rates, 2-cell rates and blastocyst rates, reduced embryo quality, and impaired development; the Coda air filter is a Effective method to remove VOC in laboratory.