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目的 :研究BMP - 4基因对C2C12细胞分化的影响。方法 :BMP - 4基因在pCI-neo质粒中构建后转入C2C12细胞。转入pCI-neo -BMP - 4重组质粒的细胞、转入pCI-neo质粒的细胞及没有转染的细胞在相同的条件下培养。结果 :转入pCI-neo质粒的细胞及没有转染的细胞在增殖期和分化期均不表达BMP - 4 ,在未分化期表现为典型的星形形态 ,并相互融合成细长而多核的肌小管。而转入pCI -neo -BMP - 4重组质粒的细胞在增殖期和分化期均大量表达BMP - 4 ,形态上不形成肌小管 ,而是形成类似成骨细胞的形态 ,并高水平表达碱性磷酸酶、在培养基中分泌骨钙素。结论 :表明将BMP - 4基因转入C2C12细胞可改变其肌源的分化途径 ,而分化为成骨细胞系 ,且这种分化转向是基因水平上的。
Objective: To study the effect of BMP - 4 gene on differentiation of C2C12 cells. Methods: The BMP - 4 gene was transferred into C2C12 cells after construction in pCI - neo plasmid. The cells transfected with the pCI-neo-BMP-4 recombinant plasmid, the plasmid transfected with the pCI-neo plasmid, and the cells without the transfection were cultured under the same conditions. Results: The cells transfected with pCI - neo plasmid and the cells without transfection did not express BMP - 4 in proliferating and differentiation stages, which showed typical star shape in undifferentiated stage and fused with each other into slender and multi - nucleated Myotubules. However, cells transfected with pCI-neo - BMP - 4 plasmid expressed BMP - 4 abundantly in both proliferative and differentiation stages, but did not form myotubes in morphology, but formed osteoblast - like morphology with high expression of basicity Phosphatase, secrete osteocalcin in culture. CONCLUSION: The BMP - 4 gene transfer into C2C12 cells can change its myogenic differentiation pathways and differentiate into osteoblast cell lines, and this differentiation is at the gene level.