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目的 探讨环孢素A对大鼠脑缺血再灌注损伤后的脑保护作用及其对IL 1β表达的影响。 方法 将 72只大鼠分为假手术组、生理盐水对照组和环孢素A治疗组 ,参照Zealonga线栓法制备局灶性脑缺血再灌注模型 ,大鼠脑缺血 2h再灌注 2 2h和 70h后 ,分别对各组各时间点大鼠进行脑TTC染色评价脑梗死体积、采用RT PCR和放免法分别对缺血区皮层IL 1β基因表达和蛋白表达进行测定。结果 各时间点环孢素A治疗组与生理盐水对照组相比 ,环孢素A治疗组脑梗死灶体积比对照组明显减小 (P <0 .0 5 ) ;环孢素A可有效降低大鼠局灶性脑缺血再灌后脑组织中IL 1β基因和蛋白的表达 (P <0 .0 1) ;与上述两组相比 ,假手术组各项观察指标则无明显异常。结论 IL 1β参与脑缺血再灌注损伤 ,环孢素A对大鼠脑缺血再灌注损伤有明显的保护作用 ,环孢素A的脑保护机制可能与抑制缺血区内IL 1β的表达有关
Objective To investigate the protective effect of cyclosporin A on cerebral ischemia-reperfusion injury in rats and its effect on the expression of IL-1β. Methods Seventy-two rats were divided into sham operation group, saline control group and cyclosporin A treatment group. The model of focal cerebral ischemia / reperfusion was established according to the Zealonga method. The rats were reperfused 2h After 70h, cerebral infarction volume was evaluated by brain TTC staining at each time point in each group. The gene expression and protein expression of IL-1β in ischemic cortex were determined by RT-PCR and radioimmunoassay. Results Compared with the control group, the volumes of cerebral infarction in cyclosporine A treated group were significantly decreased (P <0.05) at all time points compared with the control group; cyclosporine A was decreased The expression of IL-1β gene and protein in the brain tissue after focal cerebral ischemia-reperfusion in rats (P <0.01). Compared with the above two groups, there were no obvious abnormalities in the sham operation group. Conclusion IL 1β is involved in cerebral ischemia-reperfusion injury. Cyclosporine A has a protective effect on cerebral ischemia-reperfusion injury in rats. The protective mechanism of cyclosporine A may be related to the inhibition of IL 1β expression in the ischemic area