利用大鼠活体肾小管显微穿刺技术,将一种新研制的膜蛋白标记试剂直接注入近端小管腔内,用电镜放射自显影方法处理组织切片,追踪被内陷的膜蛋白,最后体视学定量分析.结果表明:在近端小管表面细胞膜和内吞泡之间存在着有效的持续不断的片段的细胞膜循环.这提示被内陷的细胞膜可以循环回到细胞表面.同时也暗示了在大鼠肾小管细胞的表面和基底面之间存在膜结构的流动和转运. A new membrane protein labeling reagent was directly injected into the proximal small lumen by using rat renal tubule micro-puncture technique. Tissue sections were processed by electron microscopy and followed by invagination of membrane proteins. The final body Quantitative analysis by visualization showed that there was an effective and sustained membrane loop between the membrane of the proximal tubule and the endocytosis, suggesting that the invaginated cell membrane could be recycled back to the cell surface, There is membrane structure flow and transport between the surface of the rat tubular cells and the basal plane.