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目的探讨体外诱导T淋巴细胞无能的条件,并分析无能T淋巴细胞的免疫生物学特性。方法以Wistar大鼠的脾细胞为刺激细胞,SD大鼠的脾细胞为反应细胞,进行混合淋巴细胞反应(MLR),分别单独或联合加入剂量不等的抗B71单克隆抗体(抗B71单抗)、抗B72单克隆抗体(抗B72单抗),制备无能T淋巴细胞。将无能T淋巴细胞和刺激细胞混合,分别加入抗CD3单克隆抗体、刀豆蛋白(ConA)及白细胞介素2(IL2),分析各种情况下无能T淋巴细胞的增殖情况;将刺激细胞与同种异体SD大鼠脾细胞混合,分别加入不同数量的无能T淋巴细胞,分析SD大鼠淋巴细胞的增殖情况。结果单独应用抗B71单抗或抗B72单抗,对淋巴细胞增殖的抑制作用不明显,而将二者联用,能明显阻断T淋巴细胞增殖;ConA及IL2能使无能T淋巴细胞增殖,而抗CD3单克隆抗体不能使无能T淋巴细胞发生增殖;无能T淋巴细胞对同种异体T淋巴细胞的增殖也产生抑制作用,该效应具有抗原特异性。结论联合抗B71单抗和抗B72单抗可诱导T淋巴细胞无能;无能T淋巴细胞在体外呈免疫无反应性,并能抑制同种异体T淋巴细胞的增殖,该抑制作用具有抗原特异性。
Objective To investigate the conditions of inducing T lymphocytes in vitro incapacity and to analyze the immunobiological characteristics of incapable T lymphocytes. Methods Splenocytes of Wistar rats were used as stimulator and splenocytes of SD rats as reaction cells. Mixed lymphocyte reaction (MLR) was performed. Anti-B71 monoclonal antibody (anti-B71 monoclonal antibody ), Anti-B72 monoclonal antibody (anti-B72 monoclonal antibody) to prepare incapable T lymphocytes. The incompatible T lymphocytes and stimulating cells were mixed and anti-CD3 monoclonal antibody, ConA and IL2 were respectively added to analyze the proliferation of incompetent T lymphocytes under various conditions; Allogeneic Sprague Dawley rats were mixed with different numbers of incompetent T lymphocytes, and the proliferation of lymphocytes in SD rats was analyzed. Results The anti-B71 monoclonal antibody or anti-B72 monoclonal antibody alone showed no obvious inhibitory effect on lymphocyte proliferation. However, the combination of both could block the proliferation of T lymphocytes. ConA and IL2 could promote the proliferation of incompetent T lymphocytes, The anti-CD3 monoclonal antibody can not make incompetent T lymphocytes proliferation; incompetent T lymphocytes on allogeneic T lymphocytes also inhibited the proliferation, the effect of antigen-specific. Conclusion The combination of anti-B71 McAb and anti-B72 McAb can induce the inability of T lymphocytes. Incompetent T-lymphocytes are immunologically non-responsive and inhibit the proliferation of allogeneic T lymphocytes. The inhibitory effect is antigen-specific.