论文部分内容阅读
目的:探讨HIV/HCV重叠感染患者外周血单核细胞亚群与肝损伤的关系。方法:观察对象为HIV/HCV重叠感染患者,分为对照组(n=11)、肝纤维化组(n=12)和肝硬化组(n=7)。运用流式细胞仪检测单核细胞及其亚群变化,瞬时弹性成像(Fibroscan)检测肝纤维化情况。比较单核细胞各亚群在不同程度肝损伤中的差异,并对HIV/HCV重叠感染患者外周血的单核细胞数与肝纤维化情况进行相关性分析。结果:HIV/HCV重叠感染患者肝硬化组与对照组比较,单核细胞CD14low CD16+和CD14high CD16+亚群显著增多(P=0.047,P=0.018)。HIV/HCV重叠感染患者肝纤维化组与对照组比较,单核细胞各亚群差异无统计学意义(P=0.84,P=0.812)。HIV/HCV重叠感染患者CD14high CD16+单核细胞与肝纤维化情况存在正性线性相关,方程成立,并且系数有统计学意义(P=0.018),方程似然比(r 2)0.45。结论:HIV/HCV重叠感染患者CD14high CD16+单核细胞增高有可能是肝损伤加重的原因之一。
Objective: To investigate the relationship between peripheral blood monocyte subsets and liver injury in patients with HIV / HCV overlap infection. Methods: The patients were divided into control group (n = 11), liver fibrosis group (n = 12) and cirrhosis group (n = 7). The changes of monocytes and their subpopulations were detected by flow cytometry, and fibrosis was detected by Fibroscan. The differences of monocytes in different degrees of liver injury were compared. The correlation between mononuclear cell count and liver fibrosis in HIV / HCV co-infected patients was also analyzed. Results: Compared with the control group, CD14low CD16 + monocytes and CD14high CD16 + monocytes were significantly increased in patients with HIV / HCV infection (P = 0.047, P = 0.018). Compared with the control group, there was no significant difference in the monocyte subsets among the HIV / HCV overlapping patients with liver fibrosis (P = 0.84, P = 0.812). There was a positive linear correlation between CD14high CD16 + monocytes and liver fibrosis in HIV / HCV co-infected patients. The equation was established and the coefficient was statistically significant (P = 0.018). The odds ratio (r 2) was 0.45. CONCLUSION: Increased CD14high CD16 + monocytes in patients with HIV / HCV infection may be responsible for the increased liver injury.