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目的建立ApoE-/-小鼠颈动脉稳定性动脉粥样硬化(AS)模型,并探讨用高场强7.0T小动物MR检测AS的形成过程。方法对10只ApoE-/-小鼠损伤并阻断一侧颈总动脉血流20 min,之后高脂饮食饲养7周,分别在术后4、7周进行7.0T MR检测与病理学检查。结果 4周后MRI显示患侧均出现不同程度的血管壁高信号,7周后MRI表现为损伤血管高壁信号区增厚并管腔狭窄、不规则,创伤后血管腔面积呈下降趋势。对应病理学检查显示,4周后颈总动脉腔内斑块形成,斑块内泡沫细胞及脂质坏死核心形成,其上覆盖厚纤维帽;7周后斑块内增生的内膜内见广泛钙质沉积。结论用损伤及高脂饮食方法可成功建立ApoE-/-小鼠稳定期AS模型;高场强7.0T MR可成功检测小鼠颈动脉稳定期粥样硬化的形成过程,进一步研究ApoE-/-小鼠易损斑块提供研究基础。
Objective To establish a stable carotid artery atherosclerosis (AS) model in ApoE - / - mice and to explore the formation of AS in high field strength 7.0T animal. Methods Ten ApoE - / - mice were injured and the common carotid artery was blocked for 20 min. After that, the high fat diet was fed for 7 weeks. The 7.0 T MR and pathological examination were performed at 4 and 7 weeks after operation. Results After 4 weeks, the MRI showed that the affected side of the ipsilateral vessels showed different degrees of vascular hyperintensity. After 7 weeks, the MRI showed thickening and stenosis of the vascular wall in the high wall of the injured vessel. The tracheal lumen area showed a decreasing trend. Corresponding to the pathological examination showed that after 4 weeks, common carotid artery plaque formation, plaque foam cells and lipid necrosis of the nucleus formed, covered with thick fiber cap; 7 weeks after plaque hyperplasia within the intima see a wide range Calcium deposition. Conclusion The stable AS model of ApoE - / - mice can be successfully established by the method of injury and high fat diet. The formation of stable atherosclerosis in carotid artery can be successfully detected by high field intensity 7.0T MR. ApoE - / - Mouse vulnerable plaque provides the basis for the study.