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牛磺酸(tau)及其相关蛋白是双螺旋丝(PHF)的主要成分,沉积于Alzheimer病(AD)脑的神经元内。Alzheimer神经纤维缠结(A-NFTs)亦被发现在实验性缺血的动物神经元中。作者通过脑解剖研究脑缺血性损害与tau免疫反应性(IR)的关系。采用8例脑梗塞后死亡者的脑标本,2例无神经性损害死亡者的脑标本做对照。脑组织切片包括梗塞坏死区及周围非坏死区,免疫组织化学染色包括:(1)甲苯紫法;(2)Bodian法;(3)Gallgas法;(4)tau、泛肽法及Alz-50免疫组化法。经组织化学处理、培养后出现深紫色的反应。 结果 缺血损害区,甲苯紫的切片上出现神经胶质反应、毛细血管增生和神经元减少;Alz-50切片上出现较多的锥体状神经元;改良的Gallgas法切片上神经元染色较淡。非坏死缺血区周围无神经胶质反应,神经元的数目及形态正常,而Alz-50切片上亦有锥体细胞,Alz-50 IR局限在神经元的核周体呈点状出现。抗
Taurine (Tau) and its related proteins are the main components of double helical filaments (PHFs) and are deposited in neurons of Alzheimer’s disease (AD) brain. Alzheimer neurofibrillary tangles (A-NFTs) have also been found in experimental ischemic animal neurons. The authors studied the relationship between cerebral ischemic damage and tau immunoreactivity (IR) by brain anatomy. Brain samples from 8 deceased patients with cerebral infarction and 2 from non-neurological damage were used as controls. Tissue sections of the brain include infarct necrosis and surrounding non-necrotic areas. Immunohistochemical staining includes: (1) Toluene violet method; (2) Bodian method; (3) Gallgas method; Immunohistochemistry. After tissue chemical treatment, dark purple reaction appeared. Results There were glomerular reaction, capillary hyperplasia and neurons in sections of Toxoplasma gondii lesion, more pyramidal neurons appeared on Alz-50 section, neuronal staining on modified Gallgas section light. No non-necrotic ischemic area around the no glial reaction, the number of neurons and normal morphology, and Alz-50 sections also have pyramidal cells, Alz-50 IR confined to the neurons in the perinuclear body appeared punctate. anti-