论文部分内容阅读
本研究利用双链RNA(double-stranded RNA,dsRNA)和非序列依赖性PCR扩增技术(sequence-independent amplification,SIA)对表现出条斑症状的感病蜀葵进行了病原鉴定,结果发现侵染蜀葵的病原为锦葵脉明病毒(Malva vein clearing virus,MVCV),这是锦葵脉明病毒在国内首次报道。为进一步明确锦葵脉明病毒中国蜀葵分离物(MVCV-AR)的来源及进化关系,克隆获得其外壳蛋白(coat protein,cp)基因(Gen Bank登录号为KX619649)。通过与其他MVCV分离物的核苷酸及氨基酸序列比对分析可知,MVCV-AR与Mexico的tomato分离物(FJ561293)的核苷酸同源性最高为90.5%;与来自Italy的DS-Ba-01分离物(FM 212972)的氨基酸同源性最高为98.3%。系统进化分析表明,MVCV-AR与DS-Ba-01分离物聚为一簇,形成独立的分支。
In this study, pathogen identification of Hollyhock in the presence of streaky symptom was carried out using double-stranded RNA (dsRNA) and sequence-independent amplification (SIA) The pathogen of hollyhock is Malva vein clearing virus (MVCV), which is the first reported in China. In order to further clarify the origin and evolutionary relationship of mallow hollyhock vetch hollyhock (MVCV-AR), the coat protein (cp) gene was cloned (Gen Bank Accession No. KX619649). By nucleotide and amino acid sequence alignment analysis with other MVCV isolates, we found that the nucleotide homology between MVCV-AR and Mexico tomato isolate (FJ561293) was 90.5% 01 isolate (FM 212972) had the highest amino acid homology of 98.3%. Phylogenetic analysis showed that the MVCV-AR and DS-Ba-01 isolates clustered into separate clusters.