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动脉硬化主要发生于大、中管径的动脉血管,尤其易发生于经常受特殊血流动力因子影响的分枝区。经由生理实验证明,动脉分枝区内皮细胞渗透性的改变会促使低密度脂蛋白(LDL)渗过内皮细胞并堆积于管壁内层,进而导致动脉硬化。然而,直接自细胞超微结构探讨动脉硬化过程中内皮细胞运送低密度脂蛋白的途径,则未有定论。本计划之目的乃利用电显追踪剂胶体金粒子(colloidal gold)与荧光追踪剂(1,1＇-dioctadecyl-3,3,3＇-tetramethylindocarbocyanine perchlo-rate(DiI)),来探讨低密度脂蛋白在高胆固醇血症大白鼠的动脉分枝区于未分枝区内皮细胞中之运送途径及在血管内壁的分布情形。
Arteriosclerosis occurs mainly in large and medium diameter arterial vessels and is particularly prone to branching areas that are often affected by specific hemodynamic factors. Physiological experiments show that the change of endothelial cell permeability in the arterial branching area will promote the penetration of low density lipoprotein (LDL) into endothelial cells and accumulate in the inner layer of the vessel wall, leading to atherosclerosis. However, the direct pathways of endothelial cell transport of LDL directly from the cellular ultrastructure of atherosclerosis have not been elucidated. The purpose of this project is to investigate the effects of low density lipids (LDIs) using colloidal gold and a fluorescent tracer (1,1’-dioctadecyl-3,3,3’-tetramethylindocarbocyanine perchlo-rate Protein transport in hyperbilirubinemic rat branches of arteries in non-branching endothelial cells and its distribution in the inner wall of blood vessels.