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目的观察日本血吸虫紫外线致弱尾蚴(UVC)疫苗免疫小鼠诱导的抗肝虫卵肉芽肿及纤维化效应。方法将60只C57BL/6小鼠随机分为UVC疫苗免疫组和感染对照组。疫苗免疫组小鼠经皮肤接种UVC后5周,每鼠攻击感染(30±2)条正常日本血吸虫尾蚴;感染对照组经皮肤感染同量尾蚴。于攻击感染后7周解剖小鼠;取肝左叶制备连续石蜡切片,测定肝脏单卵肉芽肿大小;用ELISA法检测血清透明质酸(HA)及层黏连蛋白(LN)含量,PCR-ELISA法检测肝组织TGF-β1mRNA的表达水平。结果UVC疫苗免疫组小鼠肝组织单卵肉芽肿直径为(176.25±38.67)μm,显著小于感染对照组的(304.38±53.23)μm(P<0.01),与感染对照组相比,UVC疫苗免疫组小鼠肝虫卵肉芽肿直径减小了42.10%。UVC疫苗组小鼠血清中HA、LN含量均显著低于感染对照组,肝纤维化程度明显减轻。结论UVC疫苗免疫小鼠诱导的抗肝虫卵肉芽肿及其纤维化效应同疫苗免疫诱导的细胞免疫应答的增强及高水平的IFN-γ以及肝TGF-β1mRNA表达水平的降低密切相关。
Objective To observe the anti-hepatoma egg granuloma and fibrosis induced by UVC vaccine of Schistosoma japonicum in mice. Methods Sixty C57BL / 6 mice were randomly divided into UVC vaccine immunized group and infected control group. Five weeks after inoculated with UVC, the mice immunized with vaccine were challenged with normal (30 ± 2) cercariae of Schistosoma japonicum infection. The infected control group was infected with the same amount of cercariae through the skin. The rabbits were dissected at 7 weeks after the challenge. The left lobe of the liver was used to prepare serial paraffin sections and the size of the liver single oocyte granuloma was determined. The levels of serum HA and laminin were detected by ELISA, The expression of TGF-β1 mRNA in liver tissue was detected by ELISA. Results The diameter of single-cell granuloma in mice immunized with UVC vaccine was (176.25 ± 38.67) μm, which was significantly lower than that in the control group (304.38 ± 53.23 μm, P <0.01). Compared with the control group, Group mice hepatic worm egg granuloma diameter reduced 42.10%. The serum levels of HA and LN in UVC vaccine group were significantly lower than those in infected control group, and the degree of hepatic fibrosis was significantly reduced. Conclusion UVC vaccine-induced anti-hepatomegalovirus granuloma induced by granuloma and hepatic fibrosis of the vaccine-induced immune response and enhanced high-level IFN-γ and liver TGF-β1 mRNA expression levels are closely related.