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目的利用快速导流杂交法对人乳头瘤病毒(HPV)进行快速基因分型检测,探讨黄冈地区HPV感染的型别分布及年龄特征。方法采用核酸分子快速杂交分型技术,对2014年1月-12月3 757例在黄冈市妇幼保健院就诊妇女采集宫颈脱落细胞进行DNA提取,经PCR扩增后导流杂交进行21分型检测,统计分型检测结果。结果 3 757例中HPV阳性600例,占15.97%。黄冈地区最常见的HPV亚型是52和58型,其中HPV52最高,占18.46%,其次为HPV58,占10.53%,HPV16和HPV18这2种亚型分别占9.88%和4.03%。所有病例中,主要感染年龄段在21~30岁。单一感染比例占78.67%,多重感染主要以二重感染为主。最容易与其他亚型组合感染的为HPV52型。感染亚型分布基本不受季节影响。结论研究结果反映了黄冈地区女性宫颈HPV感染的特点,亦可为地方针对性预防该病毒感染和快速诊断治疗提供数据支撑。
Objective To detect rapid genotyping of human papillomavirus (HPV) by rapid flow-through hybridization and investigate the type distribution and age characteristics of HPV infection in Huanggang area. Methods A total of 3775 cervical exfoliated cells were collected from women treated in MCH hospital in Huanggang City from January 2014 to December 2014 for DNA extraction using rapid hybridization of nucleic acid molecules. , Statistical typing test results. Results 3 757 cases of HPV-positive 600 cases, accounting for 15.97%. The most common HPV subtypes in Huanggang area are Type 52 and Type 58, among which, HPV52 is the highest, accounting for 18.46%, followed by HPV58, accounting for 10.53%. The two subtypes, HPV16 and HPV18, account for 9.88% and 4.03% respectively. In all cases, the major infection age ranged from 21 to 30 years. A single infection accounted for 78.67%, multiple infections mainly in double infection. HPV52 most easily infected with other subtypes. The subtype of infection is basically not affected by the season. Conclusions The findings reflect the characteristics of cervical HPV infection in women in Huanggang area and provide data support for targeted prevention of the virus infection and rapid diagnosis and treatment.