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目的分析乙型肝炎病毒(HBV)前S1抗原(PreS1Ag)、核心抗原(HBcAg)与HBV DNA之间的关系,探讨对判断乙肝病毒的复制与指导乙肝的治疗的意义。方法收集门诊和住院乙型病毒性肝炎患者血清标本435例,检测血清乙肝标志物(两对半)、PreS1Ag、HBcAg及HBV DNA,统计分析其中的HBV DNA≥103拷贝/ml(HBV DNA阳性)的393例。结果在HBV DNA阳性393份标本中PreS1Ag、HBcAg和HBeAg表达阳性率分别为83.7%(329/393),44.3%(174/393)与52.5%(205/393)。PreS1Ag的阳性表达率最高,与HBcAg和HBeAg的表达阳性率差异有统计学意义(P<0.05)。而HBcAg和HBeAg的表达阳性率无统计学差异(P>0.05),PreS1Ag阳性标本中的,HBcAg阳性和阴性百分率的差异有统计学意义(P<0.05)。结论检测PreS1Ag和HBcAg与HBV DNA关系密切,反映乙肝病毒复制状况。建议在传统两对半的基础上联合检测PreS1Ag和HBcAg。
Objective To analyze the relationship between preS1 antigen (HBsAg) and hepatitis B virus (HBsAg) and HBV DNA before hepatitis B virus (HBV), and to explore the significance of judging HBV replication and hepatitis B therapy. Methods The serum samples of outpatients and inpatients with viral hepatitis B were collected from 435 cases, serum HBV markers (two pairs and a half), PreS1Ag, HBcAg and HBV DNA were detected. HBV DNA ≥103 copies / ml (HBV DNA positive) Of 393 cases. Results The positive rates of PreS1Ag, HBcAg and HBeAg in 393 HBV DNA positive samples were 83.7% (329/393), 44.3% (174/393) and 52.5% (205/393), respectively. The positive rate of PreS1Ag was the highest, and the positive rate of PreS1Ag was statistically different from that of HBcAg and HBeAg (P <0.05). However, the positive rates of HBcAg and HBeAg were not statistically different (P> 0.05). There was a significant difference between the positive and negative rates of HBcAg in PreS1Ag positive samples (P <0.05). Conclusion Detection of PreS1Ag and HBcAg is closely related to HBV DNA, reflecting the status of hepatitis B virus replication. It is recommended to combine PreS1Ag and HBcAg on the basis of the traditional two and a half.