[目的]探讨硅肺患者肺灌洗液中巨噬细胞(AM)经SiO2粉尘刺激,得到的AM培养上清对肺成纤维细胞(FB)增殖的作用。[方法]收集硅肺患者支气管肺泡灌洗液中的AM,进行SiO2再次染尘,取得培养上清,刺激肺成纤维细胞。应用MTT法检测FB的增殖情况,流式细胞仪法分析FB各期细胞分布比例(即细胞增殖情况)的改变。[结果]经SiO2刺激硅肺患者AM培养上清,可使FB增殖活性增强,细胞增殖指数(proliferous index,PI)明显增加。[结论]经SiO2活化的硅肺患者肺泡AM对肺成纤维细胞有促增殖作用。 [Objective] To investigate the effect of AM supernatant on proliferation of lung fibroblasts (FB) stimulated by SiO2 dust in lung lavage fluid of silicosis patients. [Method] The AM in bronchoalveolar lavage fluid from patients with silicosis was collected, the dust was re-dyed by SiO2, and the culture supernatant was obtained to stimulate lung fibroblasts. The proliferation of FB was detected by MTT assay, and the distribution of FB (cell proliferation) was analyzed by flow cytometry. [Result] The supernatant of AM cultured with SiO2 stimulated silicosis could enhance the proliferation activity of FB and proliferous index (PI). [Conclusion] The alveolar AMs activated by SiO2 in silicosis may promote the proliferation of lung fibroblasts.