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目的优选桂芍巴布贴的提取纯化工艺。方法以挥发油得率为考察指标,采用单因素试验法考察浸泡时间、加水量、提取时间3个因素对挥发油提取效果的影响;以芍药苷和浸出物含量为综合评价指标,采用L9(34)正交试验法考察加水量、提取时间、提取次数3个因素对水提取工艺的影响。以芍药苷转移率和除杂率为考察指标,采用单因素试验法考察醇浓度对醇沉工艺的影响。结果桂芍巴布贴的挥发油最佳提取工艺为加入8倍量的水、浸泡2 h、提取5 h,此条件下得到的挥发油平均得率为0.86%;最佳水提工艺为处方量药材加10倍量水提取2次,每次1.5 h,芍药苷的平均含量为3.58 mg·g-1,RSD为1.6%,平均水浸出物含量为13.35%,RSD为1.5%。最佳纯化工艺为将提取液浓缩至相对密度1.10(60℃),加乙醇至含醇量为80%,静置24 h。结论该优选工艺可最大限度的提取有效成分,并达到了较好的去杂效果,为确定桂芍巴布贴处方的提取纯化工艺提供了实验依据。
Objective To optimize the extraction and purification process of Guishisu Babu. Methods The volatile oil yield was taken as the index of investigation. The effect of three factors on the extraction efficiency of volatile oil was investigated by single factor test. Using the content of paeoniflorin and the extract as the comprehensive evaluation index, L9 (34) Orthogonal test method was used to investigate the effects of three factors on the water extraction process, such as water addition, extraction time and extraction times. Taking paeoniflorin transfer rate and removal rate as indexes, the influence of alcohol concentration on alcohol precipitation process was investigated by single factor test. Results The optimum extraction technology of volatile oil from Guisabupaibu was: adding 8 times the amount of water, soaking for 2 hours and extracting for 5 hours, the average yield of volatile oil obtained under this condition was 0.86% The average content of paeoniflorin was 3.58 mg · g-1, the RSD was 1.6%, the average content of water extract was 13.35% and the RSD was 1.5%. The best purification process is to concentrate the extract to a relative density of 1.10 (60 ℃), add ethanol to the alcohol content of 80%, stand for 24 h. Conclusion The optimal process can extract the active ingredients to the maximum extent and achieve a better decontamination effect, which provides an experimental basis for the determination of the extraction and purification process of Guishisu Babu prescription.