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本研究用4例成人尸体延髓,年龄为19～45岁,死后2～4 h内取材。先用自来水与蒸馏水冲洗,继而以4%多聚甲醛与0.2%戊二醛0.1 mol/L磷酸缓冲液浸泡,固定8～12 h,再移入10～20%蔗糖磷酸缓冲液中10 h,冰冻切片,厚40μm。将切片分为4套,1套做对照试验,其余3套分别以兔抗5羟色胺(5-hydroxytryptamine,5-HT)、亮脑啡肽(leucine-enkephalin,L-ENK)和生长抑素(so-matostatin,SOM)特异血清做免疫组织化学反应。
In this study, 4 adult cadaver medulla oblongata, aged 19 to 45 years old, 2 to 4 h after death drawn. First with tap water and distilled water, then with 4% paraformaldehyde and 0.2% glutaraldehyde 0.1 mol / L phosphate buffer immersion, fixed 8 ~ 12 h, then transferred to 10 ~ 20% sucrose phosphate buffer for 10 h, frozen Slice, thickness 40μm. The sections were divided into four sets and one set of control experiments. The other three sets of rabbits were treated with 5-hydroxytryptamine (5-HT), leucine-enkephalin (L-ENK) and somatostatin so-matostatin, SOM) specific serum for immunohistochemical reactions.