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目的研究葫芦素C在黄瓜植株不同部位中的代谢分布;探讨不同的前处理方式对黄瓜叶中葫芦素C的影响。方法建立葫芦素C的HPLC-DAD检测方法并测定黄瓜植株不同部位中葫芦素C的量;同时将鲜嫩黄瓜叶分别进行100℃烘干、室温阴干2种不同的处理方式,检测葫芦素C量的变化;利用HPLC-Q-TOF-MS分析100℃烘干、室温阴干2种条件下鲜嫩黄瓜叶中葫芦素C的转化。结果鲜嫩黄瓜叶中葫芦素C的量最高,但随着叶变枯萎,葫芦素C的量降低;黄瓜藤中葫芦素C的量自上而下逐渐降低;黄瓜根、叶柄、雌花以及黄瓜中均未检测到葫芦素C;无论是100℃烘干还是室温阴干鲜嫩黄瓜叶,其葫芦素C的量均明显下降;在阴干的过程中,葫芦素C下降的原因可能是其转化成葫芦素苷,初步鉴定为3-葡萄糖基-葫芦素C。结论葫芦素C在黄瓜植株中不能稳定存在,鲜嫩黄瓜叶是黄瓜植株获取葫芦素C最理想的部位。
Objective To study the metabolism of cucurbitacin C in different parts of cucumber plants and to explore the effect of different pretreatment methods on cucurbitacin C in cucumber leaves. Methods The HPLC-DAD method for the determination of cucurbitacin C was established and the amount of cucurbitacin C in different parts of cucumber plants was determined. At the same time, the fresh cucumber leaves were dried at 100 ℃ and dried at room temperature for 2 different treatments, The change of cucurbitacin C in fresh cucumber leaves was analyzed by HPLC-Q-TOF-MS under the conditions of 100 ℃ drying and room-temperature drying. The results showed that cucurbitacin C content was the highest in fresh cucumber leaves, but the quantity of cucurbitacin C decreased with the leaf wilting. The content of cucurbitacin C in cucumber vine decreased gradually from top to bottom. In cucumber root, petiole, female flower and cucumber Cucurbitacin C was not detected in either fresh or dried indistinct cucumber leaves at 100 ℃. The amount of cucurbitacin C decreased significantly during the drying process. The reason for the decrease of cucurbitacin C may be due to its conversion to cucurbitacin Glycosides, initially identified as 3-glucosyl-cucurbitacin C. Conclusion Cucurbitacin C can not be stably present in cucumber plants. Cucumber leaves are the most ideal sites for cucurbitacin C in cucumber plants.