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香石竹斑驳病毒(Carnation mottle virus,CarMV)是侵染香石竹的主要病毒之一。本试验从12个香石竹品种中获得CarMV分离物,通过RT-PCR扩增包含p7、p9、CP 3个主要基因的片段,并对扩增产物进行克隆测序。通过序列比对发现CarMV的p7、p9、CP 3个基因有较高的稳定性,p7基因核苷酸序列相似性为98.10%,氨基酸序列相似性为97.81%,其中氨基酸的第11和14位存在显著差异;p9基因核苷酸序列的相似性为98.80%,氨基酸序列相似性为99.13%,氨基酸序列在第4位差异明显;CP基因核酸序列相似性为97.58%,氨基酸的相似性为98.43%,氨基酸序列的第164和331位的变异存在相关性,整个CP变异位点比较分散。证实p7和p9的变异位点主要集中在暴露与寄主互作相关的N端,推测这是导致病毒变异,与寄主互作变异的重要位点。
Carnation mottle virus (CarMV) is one of the major viruses that infects carnation. In this study, CarMV isolates were obtained from 12 carnation varieties. The fragments containing 3 major genes of p7, p9 and CP were amplified by RT-PCR, and the amplified products were cloned and sequenced. The results showed that there were high stability of p7, p9 and CP in CarMV by sequence alignment. The similarity of the nucleotide sequence of p7 gene was 98.10%, and the similarity of amino acid sequence was 97.81%. The amino acids 11 and 14 The similarity of the nucleotide sequence of p9 gene was 98.80%, the similarity of amino acid sequence was 99.13%, and the amino acid sequence was significantly different at the 4th position. The similarity of nucleotide sequence of CP gene was 97.58%, and the amino acid similarity was 98.43 %, There is a correlation between the amino acid sequence of the 164th and 331th variation, the entire CP variation sites more dispersed. It was confirmed that the variation sites of p7 and p9 mainly focused on exposing the N-terminal related to the host interaction, suggesting that this is an important site leading to the variation of the virus and the interaction with the host.