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目的建立高效液相色谱法同时测定复方醋酸泼尼松氯霉素散中醋酸泼尼松和氯霉素的含量。方法迪马ODSC18柱(4.6mm×250mm,5μm)色谱柱,以甲醇-水(60:40)为流动相,检测波长238nm,流速1.0ml·min-1,柱温30℃,进样量为20μl。结果醋酸泼尼松在2.5～15μg·ml-1范围内与吸收峰面积呈良好线性关系(r=0.9999,n=6),氯霉素在0.2～1.4mg·ml-1范围内与吸收峰面积呈良好线性关系(r=0.9999,n=6);平均回收率:氯霉素为99.5%,RSD=1.0%(n=9);醋酸泼尼松为1 0 0.9%,RSD=0.2%(n=9)。
Objective To establish a HPLC method for the simultaneous determination of prednisone acetate and chloramphenicol in compound prednisone chloramphenicol acetate. Methods The mobile phase was a Dima ODSC18 column (4.6 mm × 250 mm, 5 μm) with methanol-water (60:40) as the mobile phase. The detection wavelength was at 238 nm and the flow rate was 1.0 ml · min-1. 20 μl. Results Prednisone acetate had a good linear relationship with the absorption peak area (r = 0.9999, n = 6) in the range of 2.5 ~ 15μg · ml-1. The absorption peak of chloramphenicol in the range of 0.2-1.4mg · ml- (R = 0.9999, n = 6). The average recoveries were 99.5% for chloramphenicol and 1.0% for RSD (n = 9) (n = 9).