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目的分析和研究肿瘤坏死因子α基因(TNF-α)-308和-238位点多态性与广西煤矿工人尘肺患者遗传易感性的相关性。方法分别选择88例尘肺患者和44例粉尘接触者为研究对象,对其职业史、尘肺病史等进行问卷调查;采集每个研究对象的外周静脉血,应用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术检测TNF-α多态性。结果矽肺组和煤工尘肺组TNF-α-308和-238位点等位基因频率比对照组高,但差异无统计学意义(P>0.05)。结论 TNF-α-308和-238位点基因多态性在广西煤矿工人尘肺发病的遗传易感因素中不起作用。
Objective To analyze and study the relationship between TNF-α gene -308 and -238 polymorphisms and genetic susceptibility to pneumoconiosis in Guangxi coal miners. Methods 88 cases of pneumoconiosis and 44 cases of dust exposure were selected as the study subjects. Their occupational history and history of pneumoconiosis were surveyed. Peripheral venous blood was collected from each subject. Polymerase chain reaction-restriction fragment length The polymorphism of TNF-α was detected by PCR-RFLP. Results The frequencies of TNF-α-308 and -238 alleles in silicosis group and coal worker’s pneumoconiosis group were higher than those in control group, but the difference was not statistically significant (P> 0.05). Conclusion The genetic polymorphisms of TNF-α-308 and -238 loci have no effect on genetic susceptibility to pneumoconiosis in Guangxi coal miners.