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目的研究迪娜口服液对四氯化碳致小鼠急性肝损伤的保护作用。方法取小鼠60只,随机分成正常组,模型组,联苯双酯组,迪娜口服液小、中、大剂量组。连续给药6d后,采用0.15%四氯化碳腹腔注射(10ml/kg)复制急性肝损伤模型。禁食不禁水18h后,测定各组小鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转氨酶(aspartate transaminase,AST)和碱性磷酸酶(alkaline phosphatase,ALP)水平,观察各组小鼠肝脏组织形态变化。结果与模型组比较,各给药组小鼠血清中ALT和AST水平均显著降低,其中迪娜口服液中、大剂量组ALT水平显著低于联苯双酯组和迪娜口服液小剂量组(P<0.01)。各组小鼠血清ALP水平比较,差异均无统计学意义(P>0.05)。联苯双酯组和迪娜口服液小、中、大剂量组肝小叶结构破坏程度和水肿变性程度较模型组明显减轻,肝索排列较整齐。结论迪娜口服液对四氯化碳致小鼠急性肝损伤具有明显的保护作用。
Objective To study the protective effect of Dina oral solution on carbon tetrachloride-induced acute liver injury in mice. Methods Sixty mice were randomly divided into normal group, model group, bifendate group and Dina oral solution in small, medium and large dose groups. After 6 days of continuous administration, acute liver injury model was duplicated by intraperitoneal injection of 0.15% carbon tetrachloride (10ml / kg). After fasting for 18 hours, the levels of alanine aminotransferase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) The morphological changes of liver in each group were observed. Results Compared with the model group, the levels of ALT and AST in the serum of the mice in each treatment group were significantly decreased. The levels of ALT in the large and medium dose groups of Dina oral solution were significantly lower than those in the low dose groups of bifendate group and Dina oral solution (P <0.01). There was no significant difference in serum ALP levels among all groups (P> 0.05). Bifendate group and Dina oral liquid small, medium and large doses of hepatic lobule structure damage and degree of edema was significantly reduced than the model group, liver neatly arranged. Conclusion Dina Oral Liquid has a significant protective effect on carbon tetrachloride-induced acute liver injury in mice.