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人们认为氧化脂蛋白在动脉粥样硬化中发挥关键性作用,然而对其基本反应机制都了解甚少。我们已发现HDL可能被由过氧化物酶产生的酪氨酸基团氧化。将HDL与L-型酪氨酸,H_2O_2和辣根过氧化物酶混合,使HDL的载脂蛋白发生交联,并使与蛋白相关的荧光强度明显增加,这种反应需L-酪氨酸参与,但不依赖游离金属离子;且此反应可被过氧化氢酶或血红素毒性aminotriarole所阻断。双酪氨酸和其它酪氨酸氧化产物在被过氧化物酶/L-酪氨
Oxidized lipoproteins are thought to play a key role in atherosclerosis, yet little is known about their basic response mechanisms. We have found that HDL may be oxidized by tyrosine groups produced by peroxidases. HDL is mixed with L-tyrosine, H 2 O 2 and horseradish peroxidase to cross-link HDL apolipoproteins and increase the fluorescence intensity associated with the protein, which requires L-tyrosine Participate, but do not rely on free metal ions; and this reaction can be blocked by catalase or the heme-toxic aminotriarole. Bis-tyrosine and other tyrosine oxidation products are peroxidase / L-tyramine