The demand for high quality rice represents a major issue in rice production.The primary components of rice grain quality include appearance,eating,cooking,physico-chemical,milling and nutritional qualities.Most of these traits are complex and controlled by quantitative trait loci(QTLs),so the genetic characterization of these traits is more difficult than that of traits controlled by a single gene.The detection and genetic identification of QTLs can provide insights into the genetic mechanisms underlying quality traits.Chromosome segment substitution lines(CSSLs) are effective tools used in mapping QTLs.In this study,we constructed 154 CSSLs from backcross progeny(BC3F2) derived from a cross between ’Koshihikari’(an Oryza sativa L.ssp.japonica variety) as the recurrent parent and ’Nona Bokra’(an O.sativa L.ssp.indica variety) as the donor parent.In this process,we carried out marker-assisted selection by using 102 cleaved amplified polymorphic sequence and simple sequence repeat markers covering most of the rice genome.Finally,this set of CSSLs was used to identify QTLs for rice quality traits.Ten QTLs for rice appearance quality traits were detected and eight QTLs concerned physico-chemical traits.These results supply the foundation for further genetic studies and breeding for the improvement of grain quality. The demand for high quality rice represents a major issue in rice production. The primary components of rice grain quality include appearance, eating, cooking, physico-chemical, milling and nutritional qualities. Most of these traits are complex and controlled by quantitative trait loci ( QTLs), so the genetic characterization of these traits is more difficult than that of traits controlled by a single gene. The detection and genetic identification of QTLs can provide insights into the genetic mechanisms underlying quality traits. Chromosome segment substitution lines (CSSLs) are effective tools used in mapping QTLs.In this study, we constructed 154 CSSLs from backcross progeny (BC3F2) derived from a cross between ’Koshihikari’ (an Oryza sativa L.ssp.japonica variety) as the recurrent parent and ’Nona Bokra’ (an O.sativa L.ssp.indica variety) as the donor parent.In this process, we carried out marker-assisted selection by using 102 cleaved amplified polymorphic sequence and simple sequence repeat marke rs covering most of the rice genome .Finally, this set of CSSLs was used to identify QTLs for rice quality traits.Ten QTLs for rice appearance quality traits were detected and eight QTLs concerned physico-chemical traits.These results supply the foundation for further genetic studies and breeding for the improvement of grain quality.