目的:建立牛大力药材中高丽槐素的RP-HPLC含量测定方法。方法:采用Cosmosil5 C18-ms-Ⅱ色谱柱(4.6mm×250mm,5μm),柱温30℃;流动相为乙腈-0.1%醋酸水溶液(43∶57),流速1.0mL.min-1;检测波长为312nm;进样体积为20μL。结果:高丽槐素进样量与峰面积呈现良好的线性关系(r=0.9999),线性范围为0.13～2.0μg;平均加样回收率(n=6)为99.3%,RSD=1.5%。结论:该方法准确、快速、简便,重复性好,可用于牛大力药材的质量控制。 Objective: To establish a RP-HPLC method for the determination of cassia sophrae in Daduoli. Methods: Cosmosil5 C18-ms-Ⅱ column (4.6mm × 250mm, 5μm) was used with the column temperature of 30 ℃. The mobile phase consisted of acetonitrile-0.1% acetic acid solution (43:57) 312 nm; injection volume 20 μL. Results: There was a good linear relationship between the peak area and the amount of sophoramine (r = 0.9999). The linear range was 0.13 ~ 2.0μg. The average recovery was 99.3% (n = 6) and RSD was 1.5%. Conclusion: The method is accurate, rapid, simple and reproducible, and can be used for the quality control of Daucus herbs.