对牙齿和骨组织进行组织学研究和免疫组化观察时,关键在于组织切片的制作,切片不但要保持组织结构的完整,而且也要尽量保存组织和细胞内的抗原成分。通过免疫组化染色,能达到定性、定位乃至定量研究的目的。由于牙齿和骨组织都是钙化的硬组织,特别是牙釉质是高度钙化的硬组织, The key to histological and immunohistochemical observation of teeth and bones is to make the tissue sections. The sections should not only maintain the integrity of the tissue structure, but also keep the antigen components in the tissues and cells as far as possible. By immunohistochemical staining, to achieve qualitative, positioning and quantitative research purposes. Since both tooth and bone tissue are calcified hard tissues, especially enamel is a highly calcified hard tissue,