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目的：探讨PTEN蛋白在结肠直肠癌组织中的表达及其临床意义。方法：应用免疫组织化学技术检测1998年1月至1999年8月间于我院收治的122例结肠直肠癌组织标本中PTEN蛋白的表达。结果：在122例结肠直肠癌组织标本中有39例为阴性表达,正常结肠直肠黏膜组织、腺瘤组织均为阳性表达(P<0．01)。无论阳性面积抑或强阳性面积,PTEN蛋白表达与结肠直肠癌病人的性别、年龄无显著相关性(P>0．05)；而与结肠直肠癌周径、淋巴结转移、远处转移、肿瘤分化程度和Dukes分期存在显著相关(P<0．01)。肿瘤分化各组两两比较显示,在阳性面积中除高分化与中分化腺癌、在强阳性面积中除低分化与未分化腺癌(P>0．05)外,其余各组间统计学检验均存在显著性差异(P<0．01)。Dukes分期各期两两比较,无论是阳性面积还是强阳性面积,除B期与C期(P>0．05)外,其余各组间统计学检验均存在显著性差异(P<0．01)。结论：PTEN抑癌基因的蛋白表达低下或丢失与结肠直肠的癌变有关,可能是结肠直肠的癌变过程中一种较晚发生的事件,是进展期结肠直肠癌的一种信号。
Objective: To investigate the expression of PTEN protein in colorectal cancer and its clinical significance. Methods: Immunohistochemistry was used to detect the expression of PTEN protein in 122 cases of colorectal cancer tissues from January 1998 to August 1999 in our hospital. Results: Among the 122 cases of colorectal cancer, 39 cases were negative and the normal colorectal mucosa and adenoma were all positive (P <0.01). No correlation was found between the expression of PTEN protein and the sex and age of colorectal cancer patients (P> 0.05), but no significant correlation was found between the expression of PTEN protein and the colorectal cancer circumference, lymph node metastasis, distant metastasis, tumor differentiation There was a significant correlation with Dukes staging (P <0.01). Tumor differentiation Comparison of each group showed that, in addition to poorly differentiated and moderately differentiated adenocarcinoma in the positive area, in the strong positive area in addition to poorly differentiated and undifferentiated adenocarcinoma (P> 0.05), the remaining statistics between the groups There were significant differences (P <0.01). The Dukes staging showed no statistically significant difference (P <0.01), except for the B and C phases (P> 0.05) ). Conclusions: The low or loss of protein expression of tumor suppressor gene PTEN may be related to the colorectal carcinogenesis. It may be a later event in colorectal carcinogenesis and a signal of advanced colorectal cancer.