同工酶的研究早在五十年代就已用于植物品种鉴定工作,近年来对植物、动物及人类的各种同工酶的研究,加深了对生物形态、功能以及起源的认识。在食用菌基础理论研究上,关于同工酶的研究目前虽有开展,但是采用纯合基因型原种进行品种间食用菌同工酶的研究尚未见报道。本研究的目的在于探求利用同工酶的不同酶谱类型进行品种间鉴定,这在食用菌分类上优于只从形态特征上分类的方法,既简便又准确,对解决当前国内菌种混乱有重大意义。同时采用这种方法有助于研究食用菌基因定位等遗传规律,以指导食用菌的育种工作。本研究采用聚丙烯酰胺凝胶电泳,获得了同一菇种中 Isoenzyme studies have been used in plant identification since the 1950s. In recent years, studies on various isozymes of plants, animals and humans have deepened the understanding of biological morphology, function and origin. In the study of basic theory of edible fungi, the researches on isoenzymes have been carried out at present, however, there is no report on the study of isozyme of edible fungi among varieties with homozygous genotypes. The purpose of this study is to explore the use of isozymes of different zymogram genotypes between species identification, which is superior to the classification of mushrooms only from the morphological characteristics of the classification method, both simple and accurate, to solve the current domestic species confusion have Great significance. At the same time, this method is helpful to study the genetic rules of edible fungus gene mapping to guide the breeding of edible fungi. In this study, polyacrylamide gel electrophoresis, obtained in the same mushroom species