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目的研究牛磺酸舒张猪冠状动脉血管作用及其可能机制。方法用Powerlab离体血管环实验系统,记录KCl、组胺、5-羟色胺及细胞外Ca2+所引起的离体猪冠状动脉环的收缩,观察牛磺酸预孵对这些收缩的影响,或观察急性加入牛磺酸对持续收缩的舒张作用;观察不同药物对牛磺酸的舒血管作用的影响。结果牛磺酸(20.0 mmol/L、39.2 mmol/L、76.8 mmol/L)预孵浓度依赖性拮抗组胺(0.1 mmol/L)、5-羟色胺(10μmol/L)及细胞外Ca2+引起的猪冠状动脉环收缩。牛磺酸(20.0 mmol/L～107.6mmol/L)对KCl(30 mmol/L)所致的收缩呈现出浓度依赖性地舒张作用。去内皮和NO合成酶抑制剂L-NAME(0.1 mmol/L)对其舒张作用无影响。KCa通道抑制药四乙胺(10 mmol/L)、KATP通道抑制药格列苯脲(10μmol/L)和KIR通道抑制药氯化钡(1 mmol/L)明显抑制牛磺酸的舒血管作用,而KV通道抑制药4-氨基吡啶(1 mmol/L)无明显影响。结论在离体猪冠状动脉环,牛磺酸浓度依赖性抑制多种致痉剂引起的收缩;对持续收缩有舒张作用,该舒张作用为非内皮依赖性,可能与激动KCa、KATP和KIR通道有关。
Objective To study the effect and mechanism of taurine diastolic pig coronary artery. Methods The isolated and isolated porcine coronary artery rings induced by KCl, histamine, serotonin and extracellular Ca2 + were recorded by the Powerlab isolated vascular ring system. The effects of taurine precontraction on these contractions were observed, and the acute Adding taurine to the sustained contraction of the diastolic effect; observed different drugs on the vasodilator effect of taurine. Results Taurine (20.0 mmol / L, 39.2 mmol / L, 76.8 mmol / L) preconditioned in a concentration-dependent manner inhibited the growth of pigs induced by histamine (0.1 mmol / L), 5-HT (10 μmol / L) and extracellular Ca2 + Coronary rings contract. Taurine (20.0 mmol / L ~ 107.6 mmol / L) showed a concentration-dependent relaxation effect on contraction induced by KCl (30 mmol / L). Endothelium and NO synthase inhibitor L-NAME (0.1 mmol / L) had no effect on its diastolic function. Tetraethylamine (10 mmol / L), KATP channel inhibitor glibenclamide (10 μmol / L) and KIR channel inhibitor barium chloride (1 mmol / L) inhibited the vasodilatation of taurine significantly , While KV channel inhibitor 4-aminopyridine (1 mmol / L) had no significant effect. Conclusion In isolated porcine coronary artery rings, taurine concentration-dependently inhibits contractile responses induced by various antispasmodic agents and has a relaxation effect on sustained contraction, which is not endothelium-dependent, which may be related to the activation of KCa, KATP and KIR channels related.