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目的了解硝酸羟胺对大鼠血清丙二醛(MDA)和过氧化物歧化酶(SOD)含量的影响及其与染毒剂量的关系。方法选择Wister大鼠32只,设置硝酸羟胺50、75、100 mg/kg三个不同剂量组和生理盐水溶剂对照组,每组8只,大鼠腹腔分别注射上述剂量的硝酸羟胺和生理盐水后24 h取血,检测血清中MDA含量及SOD活力。结果大鼠血清中MDA含量随硝酸羟胺染毒剂量的增加呈上升趋势;与溶剂对照组相比,50、75、100 mg/kg剂量组MDA含量均有增加,具有显著性差异(P<0.01,P<0.05)。SOD活力随硝酸羟胺染毒剂量的增加呈下降趋势;与溶剂对照组相比,50 mg/kg剂量组虽有降低但差异不显著(P>0.05);75、100 mg/kg剂量组含量降低,具有显著性差异(P<0.01)。结论腹腔注射硝酸羟胺可引发大鼠血清中脂质过氧化反应增强、抗氧化酶含量降低,氧化损伤可能是硝酸羟胺对机体损害的主要机制。
Objective To investigate the effect of hydroxylamine nitrate on serum malondialdehyde (MDA) and superoxide dismutase (SOD) in rats and its relationship with exposure dose. Methods Thirty-two Wister rats were randomly divided into three groups: normal saline, 50, 75, 100 mg / kg hydroxylamine nitrate and normal saline. Eight rats in each group were injected intraperitoneally with the above doses of hydroxylamine nitrate and saline 24 h blood, serum MDA content and SOD activity. Results The content of MDA in serum of rats increased with the increase of the dose of hydroxylamine nitrate. Compared with the solvent control group, the content of MDA in the groups of 50, 75 and 100 mg / kg increased, with significant difference (P <0.01 , P <0.05). Compared with solvent control group, the activity of SOD decreased with the increase of the dosage of hydroxylamine nitrate injection, but the difference was not significant (P> 0.05) in the 50 mg / kg dose group, but decreased in the 75,100 mg / kg dose group , With significant difference (P <0.01). Conclusion Intraperitoneal injection of hydroxylamine nitrate can induce the increase of lipid peroxidation and the decrease of antioxidant enzyme in serum. Oxidative damage may be the main mechanism of hydroxylamine nitrate damage to the body.