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目的探讨人脐血源基质细胞(human umbilical cord blood-derived stromal cells,hUCBDSCs)联合脐血单个核细胞(human umbilical cord blood-mononuclear cells,hUCB-MNCs)共移植促进放射损伤BALB/c小鼠造血重建与植入的效应。方法BALB/c近交系小鼠经60Coγ射线8.5Gy辐照后1d按照完全随机设计分为4组,经尾静脉分别输注生理盐水、hUCB-MNCs(1×107/只)、hUCB-MNCs(1×107/只)联合hUCBDSCs(1×106/只)及hUCB-MNCs(1×107/只)联合人骨髓基质细胞(hBMSCs,1×106/只)。检测移植后各组小鼠存活率、外周血象、骨髓病理变化、成纤维细胞集落形成单位(CFU-F)和脾集落形成单位(CFU-S)计数及人源CD45+细胞植入率、归巢示踪等指标。结果采用hUCBDSCs或hBMSCs联合移植造血受抑轻,恢复快,存活率较单移植组(45%)显著提高(P<0.05),分别为75%和70%。尤其以hUCBDSCs共移植组促血小板恢复作用显著加强,并于移植后第21天恢复至照射前水平。hUCBDSCs共移植组在移植后14dCFU-F(70.40±4.34/孔)、移植后21dCFU-F(94.20±8.44/孔)和移植后12dCFU-S计数(34.75±4.50/只)、CD45+细胞植入率(34.19±2.60)%也显著高于其余组(P<0.05)。CM-DiI标记的hUCB-MNCs在体内主要归巢至骨髓和脾脏,并以hUCBDSCs共移植组小鼠骨髓中的含量(85.20±7.89)最多(P<0.05)。结论hUCBDSCs联合hUCB-MNCs共移植能加速小鼠造血重建,提高植入率。
Objective To investigate the effects of human umbilical cord blood-derived stromal cells (hUCBDSCs) co-transplantation with human umbilical cord blood-mononuclear cells (hUCB-MNCs) on the hematopoiesis in BALB / c mice Effects of reconstruction and implantation. Methods BALB / c inbred mice were randomly divided into 4 groups according to 60Coγ ray irradiation at 8.5Gy and were injected with saline, hUCB-MNCs (1 × 107 / mouse), hUCB-MNCs HBMSCs (1 × 10 6 cells / h) and hUCB-MNCs (1 × 10 7 cells / h) were combined with hUCBDSCs (1 × 10 6 cells / mouse) Survival rate, peripheral blood, pathological changes of bone marrow, CFU-F and CFU-S count and implantation rate of human CD45 + cells were detected after transplantation Tracing and other indicators. Results The hUCBDSCs or hBMSCs transplantation combined with hematopoietic stem cell transplantation showed less pain and faster recovery than the single transplantation group (45%) (P <0.05), which was 75% and 70%, respectively. In particular, hUCBDSCs co-transplantation group significantly enhanced the role of platelet recovery, and returned to the pre-irradiation level on the 21st day after transplantation. The total number of CD45 + cells implanted in the hUCBDSCs co-transplantation group at 14d after CFU-F (70.40 ± 4.34 / well), 21d CFU-F (94.20 ± 8.44 / well) and12d CFU-S (34.19 ± 2.60)% was also significantly higher than the other groups (P <0.05). CM-DiI-labeled hUCB-MNCs mainly homing to the bone marrow and spleen in vivo and the content of hUCBDSCs co-transplantation group (85.20 ± 7.89) (P <0.05). Conclusions The co-transplantation of hUCBDSCs and hUCB-MNCs can accelerate the hematopoietic reconstitution in mice and improve the implantation rate.