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目的:建立SD大鼠正畸牙移动模型,观察牙移动过程中牙周组织内降钙素基因相关肽(calcitonin gene related peptide,CGRP)的表达变化,探讨其在正畸疼痛的外周神经机制中的作用。方法:将60只SD大鼠随机分为实验组(54只)和对照组(6只)2组,实验组大鼠施加50 g力后,分别在4 h、8 h、1 d(1 d组根据据力值大小分为1 d-30 g、1 d-50 g、1 d-80 g 3个亚组)、3 d、5 d、1周、2周,随机处死6只SD大鼠,收集牙周组织,进行实时定量PCR检测,观察牙周膜内CGRP随时间及力值大小的表达变化,采用SPSS13.0软件包对数据进行统计学分析。结果:大鼠正畸牙移动过程中,牙周组织内CGRP表达随加力时间发生改变,加力4 h后开始增强,1 d后达到高峰并逐渐减弱,2周后与对照组相比无显著差异。不同力值组加力1 d后,CGRP表达随加力力值增大而升高,80 g力组>50 g力组>30 g力组。结论:正畸牙移动过程中,牙周组织内CGRP表达随加力时间增加及加力力值增大出现规律性变化,提示CGRP可能在正畸疼痛的外周神经机制中具有重要作用。
OBJECTIVE: To establish a model of orthodontic tooth movement in SD rats and to observe the expression changes of calcitonin gene related peptide (CGRP) in periodontal tissues during tooth movement and to explore its mechanism in peripheral nerve mechanism of orthodontic pain Role. Methods: Sixty Sprague-Dawley rats were randomly divided into experimental group (54 rats) and control group (6 rats). Two groups of rats were injected with 50 g force at 4 h, 8 h, 1 d The rats were randomly divided into three groups according to the size of the force: 1 d-30 g, 1 d-50 g, 1 d-80 g, 3 d, 5 d, Periodontal tissues were collected and detected by real-time quantitative PCR. The changes of the expression of CGRP in the periodontal ligament and the force value in the periodontal ligament were observed. The data were analyzed by SPSS 13.0 software package. Results: During the period of orthodontic tooth movement, the expression of CGRP in periodontal tissues changed with the force time, increased at 4 h, increased at 1 h, peaked at 1 d, and gradually decreased. After 2 weeks, Significant differences. After 1 d of different force values, the expression of CGRP increased with the increase of force, while the 80 g force> 50 g force> 30 g force. CONCLUSION: During orthodontic tooth movement, the expression of CGRP in periodontal tissues changes regularly with the increase of the force and the increase of the force, which suggests that CGRP may play an important role in the peripheral nerve mechanism of orthodontic pain.