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为挖掘烟草中α-2,7,11-西柏三烯-4,6-二醇(α-CBD)在抗肿瘤活性方面的应用价值,从烟花中提取并分离获得α-CBD,以Hep G2肿瘤细胞为材料,在体外培养条件下,分别应用噻唑蓝(MTT)法、集落形成试验和吉姆萨染色法研究了不同质量浓度(2.5~80 mg/L)的α-CBD对Hep G2细胞增殖、生长和形态变化的影响。结果发现,α-CBD具有抑制Hep G2细胞株增殖、降低细胞克隆形成并使细胞呈现凋亡形态的作用。说明烟草α-CBD具有抑制肿瘤细胞生长的活性,具有深度开发利用价值。
In order to explore the application value of α-2,7,11-cetapeptide-4,6-diol (α-CBD) in tobacco, the α-CBD was extracted and isolated from fireworks, Tumor cells were used as materials. MTT assay, colony formation assay and Giemsa staining were used to study the proliferation of Hep G2 cells in different concentration (2.5 ~ 80 mg / L) , Growth and morphological changes. The results showed that α-CBD can inhibit the proliferation of Hep G2 cell line, reduce the formation of cell clone and make the cell appear apoptotic morphology. It shows that tobacco α-CBD has the activity of inhibiting the growth of tumor cells and has the value of deep development and utilization.