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目的探讨门静脉阻断下大鼠小肠屏障功能的变化及微生物酵素对其变化的影响,从而为深入研究肠源性感染及其防治提供依据。方法将Wistar大鼠分成对照组(C),阻断组(I)和治疗组(T),每组取0、3、5、7 d4个时相点,检测血D-乳酸、二胺氧化酶(DAO)、肠道菌群微生态、组织(肠系膜淋巴结MLN、肺、肝、脾)细菌培养以及血细菌DNA等。结果与正常对照组比较,阻断组各时间点血液内D-乳酸均显著升高,C0(0.31±0.03)μg/ml与I0(1.31±0.03)μg/ml,C3(0.32±0.03)μg/ml与I3(1.50±0.14)μg/ml,C5(0.32±0.02)μg/ml与I5(1.48±0.10)μg/ml,C7(0.31±0.02)μg/ml与I7(1.08±0.43)μg/ml;血DAO均显著增高C0(0.60±0.13)U/ml与I0(1.57±0.82)U/ml,C3(0.61±0.03)U/ml与I3(1.41±0.20)U/ml,C5(0.60±0.02)U/ml与I5(1.63±0.21)U/ml,C7(0.59±0.13)U/ml与I7(1.04±0.21)U/ml;0、3 d时组织细菌培养阳性率明显增加(分别为65.00%vs0,50.00%vs0);与阻断组比较,治疗组5、7 d时血D-乳酸明显下降,治疗组5、7 d时DAO含量均显著降低,5、7 d时肠道双歧杆菌属、肠道酵母菌属明显升高。结论研究结果表明门静脉阻断可损伤大鼠小肠屏障受损,导致肠道细菌移位,引起肠源性感染。微生物酵素对门静脉阻断下小肠屏障功能有一定的改善作用,能减少肠源性感染。
Objective To investigate the changes of small intestinal barrier function in rats with portal vein occlusion and the effect of microbial enzymes on the changes, so as to provide basis for further study of intestinal infection and its prevention and treatment. Methods Wistar rats were divided into control group (C), blocking group (I) and treatment group (T). At each time point of 0, 3, 5 and 7 d, Enzyme (DAO), gut microbiota, tissue (mesenteric lymph node MLN, lung, liver, spleen) bacterial culture and blood bacterial DNA. Results Compared with the normal control group, D-lactic acid in blood was significantly increased in the blocking group at each time point. The levels of C0 (0.31 ± 0.03) μg / ml and I0 (1.31 ± 0.03) μg / ml, C3 / ml was significantly higher than that of I3 (1.50 ± 0.14) μg / ml, C5 (0.32 ± 0.02) μg / ml vs I5 (1.48 ± 0.10) μg / ml, C7 / ml; blood DAO were significantly increased in C0 (0.60 ± 0.13) U / ml and I0 (1.57 ± 0.82) U / ml, C3 0.60 ± 0.02 U / ml and I5 1.63 ± 0.21 U / ml, C7 0.59 ± 0.13 U / ml and I7 1.04 ± 0.21 U / ml. The positive rates of bacterial culture in 0 and 3 d groups were significantly increased (65.00% vs0, 50.00% vs0 respectively). Compared with the blocking group, the blood D-lactate decreased significantly on the 5th and 7th day in the treatment group, while the DAO content in the treatment group decreased significantly on the 5th and 7th day Bifidobacterium intestinal, intestinal Yeast significantly increased. Conclusion The results show that portal vein injury can damage the small intestine barrier in rats, resulting in intestinal bacterial translocation, causing intestinal infection. Microbial enzymes on the portal vein barrier function of the small intestine have some improvement, can reduce intestinal infection.