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为观察牛主动脉中蛋白聚糖对培养的人脐静脉内皮细胞生长的影响及其在动脉粥样硬化形成中的作用，用解聚提取、离子交换及凝胶过滤柱层析法从牛主动脉内、中膜分离出硫酸乙酰肝素蛋白聚糖、硫酸软骨素蛋白聚糖和硫酸皮肤素－硫酸软骨素蛋白聚糖。用氚标胸腺嘧啶脱氧核苷掺入法和细胞计数法观察上述三种蛋白聚糖对培养的第一代人脐静脉内皮细胞生长的影响。结果发现，用氚标胸腺嘧啶脱氧核苷掺入法计算硫酸乙酰肝素蛋白聚糖的抑制率（醛酸含量２．１～８．３ｍｇ／Ｌ）分别为２８．３％、５２．３％和７５．４％，硫酸软骨素蛋白聚糖的抑制率（醛酸含量９．５～３７．８ｍｇ／Ｌ）分别为６１％、８３％和９１％，硫酸皮肤素－硫酸软骨素蛋白聚糖的抑制率（醛酸含量９．３～３６．９ｍｇ／Ｌ）分别为５８．４％、８２．４％和８６％。用细胞计数法计算硫酸乙酰肝素蛋白聚糖（醛酸含量２．１～８．３ｍｇ／Ｌ）的抑制率分别为２１．５％、３７．８％、６２．９％，硫酸软骨素蛋白聚糖（醛酸含量９．５～３７．８ｍｇ／Ｌ）的抑制率分别为３７．２％、６８．９％和７６．５％，硫酸皮肤素硫酸软骨素蛋白聚糖（醛酸含量９．３～３６．９ｍｇ／Ｌ）的抑制率分别为４４．５％、６１．６％和７１．７％。结果?
To observe the effect of proteoglycan in bovine aorta on the growth of cultured human umbilical vein endothelial cells and its role in the formation of atherosclerosis, Arterial, medial separation of heparan sulfate proteoglycan, chondroitin sulfate proteoglycan and dermatan sulfate - chondroitin sulfate proteoglycan. The effects of the three proteoglycans on the growth of cultured human first-generation human umbilical vein endothelial cells were observed by tritiated thymidine incorporation and cell counting. The results showed that the inhibitory rate of heparan sulfate proteoglycan (n-acid content 2.1 ~ 8.3mg / L) was 28.3%, 52.3% and 75.4%, the inhibitory rate of chondroitin sulfate proteoglycan (albumen acid content 9.5 ~ 37.8mg / L) were 61%, 83% and 91% respectively. The inhibitory rate of dermatan sulfate-chondroitin sulfate proteoglycan The inhibitory rate (aldehyde content 9.3 ~ 36.9mg / L) was 58.4%, 82.4% and 86% respectively. The inhibitory rates of heparan sulfate proteoglycan (21.5%, 37.8%, 62.9%, respectively) calculated by cell counting method were The inhibitory rates of sugar (albumen acid content 9.5 ~ 37.8mg / L) were 37.2%, 68.9% and 76.5% respectively. The inhibitory rates of dermatan sulfate-chondroitin sulfate proteoglycan .3 ~ 36.9mg / L) were 44.5%, 61.6% and 71.7%, respectively. result?