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目的:优选从西洋参茎叶总皂苷中分离纯化拟人参皂苷F11的最佳工艺。方法:以拟人参皂苷F11(PF11)提取率为指标,建立HPLC-ELSD检测PF11的方法,并优选AB-8大孔吸附树脂纯化西洋参茎叶总皂苷中PF11的工艺。结果:最佳工艺为西洋参茎叶总皂苷过10倍量AB-8大孔吸附树脂柱,流速5 mL.min-1,依次用7.5 BV 30%～33%乙醇,8 BV 35%～40%乙醇进行洗脱,收集35%～40%乙醇洗脱液,回收乙醇,蒸干,得PF11粗品纯度>50.0%。结论:大孔吸附树脂分离拟人参皂苷F11方法简单、快速、可行。
Objective: To optimize the isolation and purification of pseudoginsenoside F11 from the total saponins of American ginseng stem and leaf. Methods: The method of HPLC-ELSD for the determination of PF11 was established based on the extraction rate of pseudo-ginsenoside F11 (PF11), and the purification of PF11 from the total saponins of American ginseng by AB-8 macroporous adsorption resin was optimized. Results: The optimum conditions were as follows: the volume of AB-8 macroporous resin was 10 times of the total saponin of American ginseng stem and leaf, the flow rate was 5 mL.min-1, the medium was 7.5 BV 30% -33% ethanol, Ethanol elution, collecting 35% to 40% ethanol eluent, ethanol recovery, evaporated to give crude PF11> 50.0% purity. Conclusion: The method of macroporous resin separation of pseudo-ginsenoside F11 is simple, rapid and feasible.