应用马来丝虫成虫冰冻切片抗原免疫酶染色技术(IEST),检测班氏丝虫微丝蚴阳性病人107例及治疗后一年微丝蚴阴转者35例和治后三年者112例血清特异IgG、IgE和IgM抗体水平,结果表明:治前病人血清IgG抗体滴度≥1:40者阳性率为96.3％,阳性GMRT为116.6;IgE抗体滴度≥1:20者阳性率为86.9％,阳性GMRT为37.1;IgM抗体滴度≥1:20者阳性率为88.8％,阳性GMRT为31.0,该三种抗体从阳性滴度 Application of frozen sections of Malayan worm antigen immunohistochemical staining (IEST), detection of Bancroftian filarial microfilaria positive in 107 patients and treatment of microfilaria females one year after the transfer of 35 cases and 112 cases of three years after treatment Serum specific IgG, IgE and IgM antibody levels, the results showed that: pre-treatment patients with serum IgG antibody titer ≥ 1:40, the positive rate was 96.3%, the positive GMRT was 116.6; IgE antibody titer ≥ 1:20, the positive rate was 86.9 %, The positive GMRT was 37.1; IgM antibody titer≥1: 20, the positive rate was 88.8%, the positive GMRT was 31.0, the three antibodies from the positive titers