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目的:提高完善妇宁丸质量标准。方法:采用显微法对益母草、党参、地黄、当归、茯苓和黄芩进行鉴别;采用TLC法对香附、陈皮和甘草进行鉴别;采用HPLC法测定白芍中芍药苷的含量,色谱柱:Agilent Zorbax XB-C_(18)(250 mm×4.6 mm,5μm),流动相:乙睛-水(13:87);柱温:35℃,流速:1.0 ml·min~(-1),检测波长:230 nm。结果:益母草、党参、地黄、当归、茯苓和黄苓的显微特征较易见,TLC色谱斑点清晰,专属性强,重复性良好。芍药苷对照品在0.21～4.71μg范围内线性关系良好(R~2=0.999 7),平均回收率99.25%,RSD为0.21%(n=6)。结论:本方法简便、可靠、准确,灵敏度高,重复性好,可用于该制剂的质量控制。
Objective: To improve and perfect the quality standard of Fuming pill. METHODS: Leonurus, Codonopsis, Radix Rehmanniae, Angelica, Poria and Radix Scutellariae were identified by microscopic method. TLC, HPLC and HPLC were used to determine the content of paeoniflorin in Radix Paeoniae Alba, the column was Agilent The mobile phase was acetonitrile-water (13:87). The column temperature was 35 ℃ and the flow rate was 1.0 ml · min -1. The detection wavelength : 230 nm. Result: The microscopic features of Motherwort, Codonopsis pilosula, Radix Rehmanniae, Angelica, Poria and Radix Ligusticilli were more obvious. TLC chromatogram spots were clear, specific and reproducible. The calibration curve of paeoniflorin showed a good linearity (R ~ 2 = 0.999 7) in the range of 0.21 ~ 4.71 μg with an average recovery of 99.25% and a RSD of 0.21% (n = 6). Conclusion: The method is simple, reliable, accurate, sensitive, reproducible and can be used for the quality control of the preparation.