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研究了9μg/L暴毒浓度的镉(Cd2+)、铅(Pb2+)和1.08μg/L暴毒浓度的汞(Hg2+)对泥蚶鳃和消化腺组织显微结构和超微结构的影响。实验处理时间为28d。3种重金属胁迫下鳃组织显微结构观察显示鳃丝排列混乱,血细胞堆积现象严重;超微结构观察发现,Cd2+和Hg2+胁迫下鳃细胞发生细胞核皱缩,有大量的线粒体和溶酶体。Pb2+攻毒组的鳃细胞中聚集大量的嗜锇性物质。3种重金属胁迫下消化腺组织显微结构显示消化腺细胞破碎,完整度变差;从超微结构来看,在Cd2+和Pb2+的胁迫下,消化腺细胞中出现嗜锇性物质;另外在Pb2+胁迫下,消化腺细胞中有溶酶体;然而在Hg2+胁迫下,泥蚶消化腺细胞受到的影响不明显。本研究推测在重金属胁迫下,泥蚶可能会通过溶酶体和重金属形成不溶性颗粒,从而将重金属隔离进行解毒作用;大量产生的线粒体可能对重金属引起的氧化胁迫进行呼吸补偿反应。
The effects of cadmium (Cd2 +), Pb2 + and Hg2 + at a concentration of 9μg / L on the gill and digestive gland tissues were studied. Experimental treatment time is 28d. Under the stress of heavy metals, the microstructure of gill tissues showed that the gill filaments were arranged in a disorderly manner and the hematopoiesis was serious. Ultrastructural observation showed that there were numerous mitochondria and lysosomes in gill cells under Cd2 + and Hg2 + stress. Pb2 + challenge group gill cells gathered a large number of osmiophilic substances. Under the stress of Cd2 + and Pb2 +, the osmiophilic substances appeared in the digestive gland cells under the stress of Cd2 + and Pb2 +. Under the Pb2 + stress , There are lysosomes in the digestive gland cells; however, under the stress of Hg2 +, the effects of digestive gland cells in the muddy suet are not obvious. In this study, it is speculated that under heavy metal stress, Sputnik may form insoluble particles through lysosomes and heavy metals, so that heavy metals are isolated and detoxified. A large amount of mitochondria may undergo respiratory compensation reaction to oxidative stress caused by heavy metals.