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近年来,我们对采集的野生猴头菇经冷冻处理后再进行组织分离,取得了良好效果,现简介如下:(一)材料:分离材料是从长白山区安图县采集的野生猴头,将其分成两组,一组经冰箱(0℃以下)保存20天后进行组织分离;另一组不经冷冻直接进行组织分离(对照)。培养基为马铃薯、葡萄糖加富培养基。(二)方法:先将分离材料用70%的酒精棉球进行表面消毒后放入无菌培养皿内,再置接种箱中,按无菌操作将菇体掰成两半或纵切一刀,用解剖刀在切面中部挖取黄豆
In recent years, we collected the wild Hericium freeze-treated and then tissue separation and achieved good results, are summarized as follows: (a) material: separation material is collected from the Antang County, Changbai Mountains wild Hericium will They were divided into two groups. One group was subjected to tissue separation after being stored in a refrigerator (below 0 ° C) for 20 days. The other group was directly subjected to tissue isolation without freezing (control). The medium is potato, glucose plus rich medium. (B) Methods: The first separation of materials with 70% alcohol cotton ball surface disinfection into sterile petri dishes, and then placed inoculation box, according to aseptic operation of the mushroom body breaking in half or slitting a knife, Use a scalpel to dig soybeans in the middle of the cut