【摘 要】
：
This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its acti-vation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was
【机 构】
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College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;College of Fo
【出 处】
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浙江大学学报（英文版）（B辑：生物医学和生物技术）
论文部分内容阅读
This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its acti-vation effects on RAW264.7 macrophages. It showed that the obtained G. chouae polysaccharide (GCP-3A) was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. GCP-3A was composed of xylose, galactose, glucose, and mannose with a molar ratio of 3.00:29.28:0.63:0.45, and it containedα,β-glycosidic linkages. Scanning electron microscopy (SEM) and a Congo red test showed that it was a heterogeneous polysaccharide with irregular interwoven sheets and rods, and did not have a triple-helix conformation. Furthermore, GCP-3A significantly promoted the proliferation of RAW264.7 macrophages and the secretion of nitric oxide (NO) in tests of 3-(4,5-dimethylthiahiazo-2-yl)-2,5-diphenytetrazoliumromide (MTT) and NO.
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