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采用外标法在 C18柱上用 HPLC 色谱技术测定7-ACA 含量,在10min 内达到基线分离,分离度大干1500,此方法回收率高,重现性好,在1.5—25μg/ml 的范围内有很好的线性关系,相关系数大于0.9990。
The external standard method was used to determine the content of 7-ACA on C18 column by HPLC chromatography, and the baseline was separated within 10 minutes with a resolution of 1,500. The method has high recovery and good reproducibility. The range of 1.5-25μg / ml There is a good linear relationship, the correlation coefficient greater than 0.9990.