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通常认为胃肠道粘膜下神经节可能是感受胃肠粘膜刺激的感觉神经元或支配胃肠粘膜的运动神经元或兼而有之。近年来应用免疫组织化学染色技术发现:豚鼠小肠粘膜下神经节的神经细胞中含有多种脑肠肽以及合成乙酰胆碱所必需的酶的免疫反应活性,例如胆囊收缩素(CCK)、神经肽Y(NPY)、生长抑素(SOM),P物质(SP)、血管活性肠肽(VIP)、胆碱乙酰基转移酶(ChAT)的免疫活性。Furness等用相互间无交叉反应的高度特异性抗体同时标记豚鼠小肠粘膜下神经元中的不同抗原,再以荧光标记的高度特异性第二抗体标记第一抗体,研究了该神经胞体内各标记神经化学物质的共存情况。发现豚鼠小肠粘膜下神经节中神经元可分二大类:45%神经胞体中仅有VIP免疫反应性,主要集中在神经节中心,其神经末梢可能
Gastrointestinal submucosal ganglia are generally considered to be sensory neurons that sense gastrointestinal mucosal stimulation or motor neurons that dominate the gastrointestinal mucosa. In recent years, the application of immunohistochemical staining techniques found that: the guinea pig small intestinal submucosal ganglia nerve cells contain a variety of brain gut peptide and acetylcholine synthesis necessary for the enzyme activity of the immunoreactive, such as cholecystokinin (CCK), neuropeptide Y ( NPY, SOM, SP, VIP and ChAT. Furness and other highly specific antibodies without cross-reaction with each other simultaneously labeled different antigens in guinea pig small intestinal submucosal neurons, and then the first antibody labeled with a fluorescently-labeled highly specific secondary antibody to study the neuronal somatic markers Coexistence of neurochemicals. Found that guinea pig intestinal submucosal ganglia neurons can be divided into two categories: 45% of neuronal cells only VIP immunoreactivity, mainly in the center of the ganglion, the nerve endings may