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目的 探讨逆转人肺癌耐药系HTB - 5 6R耐药性的可行性。方法 采用MTT比色法研究钙通道阻滞剂维拉帕米和人类重组γ -干扰素对DDP细胞毒性在人类肺癌细胞系HTB - 5 6R上的的影响。结果 γ -干扰素与维拉帕米能部分恢复HTB - 5 6R对顺铂 (DDP)的敏感性。当维拉帕米浓度 >4mg/L时 ,能明显增加DDP的细胞毒性 ,耐药株的抑制率 >19.0 % (P <0 .0 5 )。γ -干扰素浓度 >4.0× 10 6 U/L时 ,也能达到此效果 ,细胞抑制率 >3 6.9% (P <0 .0 5 )。联合两药比单独使用有更好的效果 ,维拉帕米为 2mg/L、γ -干扰素为 2 .0× 10 6 U/L时 ,细胞抑制率就达 3 6.9% (P <0 .0 1)。不论单独使用还是联合使用逆转效果均呈剂量效应。结论 γ -干扰素与维拉帕米能逆转HTB - 5 6R的耐药性 ,而且联合使用有更好的效果。
Objective To investigate the feasibility of reversing drug resistance of human lung cancer cell line HTB - 5 6R. Methods The effects of verapamil, a calcium channel blocker, and human recombinant γ - interferon on the cytotoxicity of DDP on human lung cancer cell line HTB - 5 6R were studied by MTT assay. Results γ - interferon and verapamil partially restored the sensitivity of HTB - 5 6R to cisplatin (DDP). When verapamil concentration> 4mg / L, can significantly increase the cytotoxicity of DDP, drug-resistant strains inhibition rate> 19.0% (P <0.05). This effect was also achieved when the concentration of IFN - γ> 4.0 × 10 6 U / L, with a cell inhibition rate of 3 6.9% (P <0.05). Combination of two drugs have better effect than single use, verapamil 2mg / L, γ - interferon is 2.0 × 10 6 U / L, the cell inhibition rate reached 3 6.9% (P <0. 0 1). Either alone or in combination, the reversal effect was dose-effect. Conclusion γ - Interferon and verapamil can reverse the drug resistance of HTB - 5 6R, and the combined effect is better.