Leaves of fine Populus tomentosa genotype TC152 were used as explants to establish cell suspension lines. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg·L-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg·L-1 2,4-D. The best subculture medium was MS + 0.8 mg·L-1 2,4-D + 30 g·L-1 sucrose with a subculture cycle of seven days. The effects of plant growth regulators on callus induction and establishment of cell suspension lines were studied. The callus induction rate was the highest on a MS solid medium supplemented with 1.0 mg · L-1 2,4-D. A cell suspension line could be obtained by inoculating calli which were not subcultured into a MS liquid medium supplemented with 1.5 mg · L-1 2,4-D. The best subculture medium was MS + 0.8 mg · L-1 2,4-D + 30 g · L-1 sucrose with a subculture cycle of seven days.