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目的探讨补阳还五汤对局灶性脑缺血大鼠脑组织TLR4表达的影响。方法 72只大鼠随机分为假手术组、模型组和补阳还五汤组,每组按给药后7、14、21 d 3个时间点再各分3组,用免疫组化、Western blot、RT-PCR法检测各组不同时间点TLR4的蛋白和基因表达。结果模型组和补阳还五汤组各时间点TLR4蛋白和mRNA表达均高于假手术组(P<0.05),补阳还五汤组在第7、14天TLR4蛋白表达明显低于模型组,但TLR4mRNA水平在第7天、14天却明显高于模型组,差异均有统计学意义(P<0.05)。结论补阳还五汤通过调节缺血后脑组织TLR4的表达可能是其治疗脑缺血的作用机制之一。
Objective To investigate the effect of Buyang Huanwu Decoction on TLR4 expression in brain tissue after focal cerebral ischemia in rats. Methods Seventy-two rats were randomly divided into sham operation group, model group and Buyang Huanwu Decoction group. Each group was divided into 3 groups at 7, 14, and 21 d after the administration. Each group was divided into 3 groups by immunohistochemistry, The expression of TLR4 protein and gene was detected by RT-PCR and Western blotting at different time points. Results The expressions of TLR4 protein and mRNA in model group and BYHWD group were higher than those in sham operation group at each time point (P <0.05). The expression of TLR4 protein in BUYH group at 7 and 14 days was significantly lower than that in model group , But the level of TLR4 mRNA on day 7 and 14 was significantly higher than that of model group (P <0.05). Conclusion Buyang Huanwu Decoction can regulate the expression of TLR4 in the brain tissue after ischemic reperfusion, which may be one of its mechanisms in the treatment of cerebral ischemia.