目的探讨丝切蛋白-1的表达对人皮肤鳞状细胞癌细胞增殖及迁移能力的影响。方法丝切蛋白-1特异性小干扰RNA(siRNA)转染人皮肤鳞状细胞癌A431细胞;实时定量PCR和Western blot检测丝切蛋白-1表达,CCK-8法检测细胞增殖活性,细胞划痕试验检测细胞的迁移能力。结果靶向抑制丝切蛋白-1后,A431细胞丝切蛋白-1表达水平下降,同时细胞增殖活性降低(P<0.05),划痕试验结果显示细胞迁移愈合能力下降。结论抑制丝切蛋白-1表达可降低皮肤鳞状细胞癌细胞的活性与迁移能力,可作为皮肤肿瘤防治的靶点。 Objective To investigate the effects of cofilin-1 expression on proliferation and migration of human skin squamous cell carcinoma cells. Methods Human cutaneous squamous cell carcinoma A431 cells were transfected with cotin-1 specific small interfering RNA (siRNA). Cofilin-1 expression was detected by real-time PCR and Western blot. The cell proliferation activity, Trace test to detect cell migration ability. Results The results showed that the expression of cofilin-1 in A431 cells was decreased and the cell proliferation activity was decreased (P <0.05). The results of scratch test showed that the ability of cell migration and healing decreased. Conclusion Inhibition of cofilin-1 expression can reduce the activity and migration ability of cutaneous squamous cell carcinoma cells, which can be used as a target of skin tumor prevention and treatment.