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应用ELISA双抗体夹心法检测了45例各种胸水中TNFα的含量,并以血清TNFα含量作对照研究。结果发现脓性胸水TNFα含量均高于其他组(P<0.05);结核性胸水高于漏出液组(P<0.05)和癌性胸水组(P<0.05),低于脓性组(P<0.05),癌性胸水组和漏出液组相差无显著意义(P>0.05);而低于脓性胸水组和结核性胸水(P<0.05),漏出液组最低,显著低于其它各组(P<0.05)。血清TNFα含量和胸水TNFα含量存在若明显的相关关系(r=0.675,P<0.01)。此外,胸水TNFα含量还和胸水的中性粒细胞数存在着显著的相关关系(r=0.426,P<0.05)。结果提示胸水TNFα参与炎症反应的过程,而且测定胸水TNα有助于鉴别胸水的性质。
The levels of TNFα in 45 cases of various pleural effusions were detected by ELISA double antibody sandwich method. The content of serum TNFα was used as a control study. The results showed that the content of TNFα in purulent pleural effusion was higher than that in other groups (P<0.05); tuberculous pleural effusion was higher in the leakage fluid group (P<0.05) and cancerous pleural effusion group (P<0.05) than in the purulent group (P< 0.05) There was no significant difference between the cancerous pleural effusion group and the leakage fluid group (P>0.05), but lower than the purulent pleural effusion group and the tuberculous pleural effusion (P<0.05). The leakage fluid group was the lowest, significantly lower than other groups ( P<0.05). There was a significant correlation between serum TNFα content and thoracic water TNFα content (r=0.675, P<0.01). In addition, there was a significant correlation between TNFα content in pleural effusion and neutrophils in pleural fluid (r=0.426, P<0.05). The results suggest that pleural effusion TNFα participates in the process of inflammatory reaction, and the determination of thoracic water TNα is helpful to identify the nature of pleural effusion.